Effect of resveratrol on mouse ovarian vitrification and transplantation

Abstract Background After ovarian tissue transplantation, ischemia-reperfusion injury and free radicals cause follicle depletion apoptosis. Therefore, the use of antioxidants to reduce production is an important method address consequences injury. Resveratrol a natural active polyphenol compound with anti-inflammatory, antitumor, strong antioxidant anti-free radical properties. The aim this study was investigate whether resveratrol could improve effect autologous transplantation after cryopreserve-thawn mouse tissue. Methods Whole-ovary vitrification autotransplantation models were used effects resveratrol. Six-week-old female mice from Institute Cancer Research (ICR) subjected vitrification. All ovaries preserved in liquid nitrogen for 1 week before being thawed. thawing, tissues autotransplanted bilateral kidney capsules. Mice ( n = 72) randomly divided into four groups determine optimal concentration (experiment I). Treatments given as follows: saline, 5 mg/kg resveratrol, 15 45 which administered orally one week. Grafted collected analysis on days 3, 7, 21 transplantation. Ovarian morphology assessed by hematoxylin eosin staining. Serum FSH E 2 levels measured estimate transplanted reserve endocrine function. Other two groups—saline further evaluate explore mechanisms underlying II). apoptosis terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assays. Immunohistochemistry, qRT-PCR western blotting (MDA, SOD, NF-κB, IL-6 SIRT1) Moreover, oocytes derived at cultured fertilized vitro. Results proportions morphologically normal (G1) follicles 7 significantly higher group than saline group. TUNEL-stained (%) decreased compared Western blot revealed that SOD2 SIRT1 day MDA NF-κB lower 3. Likewise, 7. These results are basically consistent results. In addition, mean number retrieved fertilization cleavage increased Conclusions Administration quality cryopreserved embryo outcome, through anti-inflammatory antioxidative mechanisms.